Composite

Part:BBa_K1765003:Design

Designed by: Kinuko Ueno   Group: iGEM15_Tokyo-NoKoGen   (2015-09-11)


Pconst-colE1-T0 termiinator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1598
    Illegal NheI site found at 1621
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 300


Design Notes

There is no restriction enzyme sites in the insert.


Source

It comes from genomic sequence of E.Coli, and synechocystis p. PCC 6803.

References